Cause: Time based Cookie Insert persistence works as expected when a client from the same time zone makes a connection. However, when the client machine and NetScaler appliance are in different time zones, the cookie is not valid. For example, when a client in EST time zone sends a cookie at 11:00 AM EST to a NetScaler appliance in the PST time zone, the appliance receives the cookie at 2:00 PM PST. As a result of the difference in time, the cookie is not valid, and the connection is immediately timed-out.
Resolution: Set the time-out value for Cookie Insert persistence to 0.